IDEXX SNAP BVDV Antigen Test [25ct]

Snap Bovine Viral Diarrhea Virus (BVDV) Antigen Test allows for on-farm testing and confirmation of bovine diarrhea virus infected cattle in 20 minutes. This easy-to-use snap test requires serum from a blood sample collection or ear-notch tissue sample. Ideal for testing newborn calves, replacements and bulls. Also identifies persistently infected animals in the herd. Highly sensitive and specific results—enzyme-linked immunosorbent assay (ELISA) technology. Allows the producer to quickly determine positive cases and persistently infected animals for a healthier herd. Ideal for use on farm or veterinary clinic use.

INSTRUCTIONS:
Allow all components to come to room temperature.

Ear notch:

• Dispense 1.5 ml of conjugate into a vial containing a single ear notch.
• Cap vial and swirl gently to soak ear notch.
• Incubate vial at room temperature (18 to 30 °C) for at least 10 minutes (range: 10 to 60 minutes).
• Pour liquid contents of the vial into the sample well of the SNAP device.
• When color first appears in the activation circle (may take 15 to 60 seconds), press firmly to activate. You will hear a distinct ‘snap.’ Note: some liquid may remain in the sample well.
• Wait 10 minutes, then read results (range: 10 to 15 minutes).

Serum test: A heating device is needed (such as a heater block, water bath, etc.) capable of maintaining a temperature of 45 +/- 5 °C

• Dispense 3 drops (150 µL) of serum sample into a sample tube; using a fresh pipette, dispense 6 drops (200 µL) of conjugate into the sample tube.
• Cap the sample tube and gently invert 3 to 5 times to mix.
• Place the sample tube in a heater block and incubate for 10 minutes at 45 °C (range: 10 to 20 minutes).
• Pour the contents of the sample tube into the sample well of the SNAP device.
• When color first appears in the activation circle (may take 15 to 60 seconds), press firmly to activate. You will hear a distinct ‘snap.’ Note: some liquid may remain in the sample well.
• Wait 10 minutes, then read results (range: 10 to 15 minutes).

IDEXX SNAP BVDV Antigen Test (25 Count) Highlights:

• SKU: 23612
• Manufacturer: Idexx
• Do not perform assay in direct sunlight.
• Helps prevent the spread of BVDV in your herd
• Do not reuse pipettes which were in contact with a specimen
• Do not mix components from kits with different serial numbers
• Do not use a SNAP device activated prior to the addition of sample
• Reduces the risk of introducing a persistently infected animal into the herd
• Do not pipette conjugate from its bottle using a pipette which was in contact with a specimen
• Always use one hand to stabilize SNAP BVDV device during activation step, to prevent splashing
• Do not cross-contaminate sample collection device(s). Soak in disinfectant before and after collection
• Betadine cannot be used as a disinfectant when obtaining ear notches as this will interfere with the assay
• If stored in a refrigerator, allow all components to equilibrate at 18 to 30 °C for at least 30 minutes before use
• Helps reduce losses caused by BVDV such as still births and abortions, poor milk quality, low volume, and poor performance
• Test can be run with small or large ear-notch samples or serum samples. Idexx recommends choosing a large ear-notch sample, as this sample type delivers results in 20 minutes without any interference from maternal antibodies. Refer to the product insert for more information